Armadillo BAC Library
The armadillo (Dasypus novemcintus) BAC library has been
constructed by Wendy Lippmann in Chris
Amemiya’s laboratory at the Virginia Mason Research
Center (VMRC). This library is designated VMRC-5. Blood from
a male armadillo was obtained from Dr. Jim Loughry, Valdosta
State University, Georgia. Preparation of the library
closely followed the cloning approach developed in Pieter
de Jong’s laboratory (Osoegawa
et al., 1998). DNA fragments from the appropriate size
fraction were cloned into the pCC1BAC vector (Epicentre
Technologies). The ligation products were then transformed
into DH10B (T1 resistant) electro-competent cells (Invitrogen).
The library has been arrayed into 384-well microtiter dishes.
This work was funded by NIH grants HG02526-01 (Chris T. Amemiya,
VMRC) and HG025323-01 (Pieter J. de Jong, CHORI).
Please note: The following data is provisional pending further
VMRC-5 Armadillo (Dasvous novemcintus) BAC
Provisonal Data, posted March 18, 2003.
|Total 384-well dishes
|Insert Size Average
for a legend of the previous tables. Data on the VMRC-5 clone
average insert size has been determined by Pulsed Field Gel
Electrophoresis (Not yet available).
Hybridization filters can be ordered either per entire set
of 12 filters or the first (or last) segment with 6 filters
(price per set is either 12 or 6 times $200).
Shipping will be done by Federal Express and charged to recipient's
account number. With respect to the scheduling of shipments
of arrayed library copies, we would like to make you aware
of our policy
for array distribution.
Please visit the ordering
information page if you want to place an order.
Academic and commercial users interested in a copy of the
BAC library should contact Pieter J. de Jong (firstname.lastname@example.org,
fax: (510) 450-7924). For hybridization
membranes, please contact BACPAC
Please contact Pieter
J. de Jong for questions related to this library.