| VMRC-7
Bat BAC Library |
 |
The bat BAC library was constructed by Alicia Hill-Force,
Tsutomu Miyake and Joshua Danke in Chris
Amemiya’s laboratory at the Virginia Mason Research
Center (VMRC). This library was designated VMRC-7. Brain tissue
was obtained from a male Greater Horsehoe Bat (Rhinolophus
ferrumequinum) kindly provided from Dr. Walter Metzner, University
of California, Los Angeles, CA. Brain cells were embedded
in Incert agarose and high molecular weight DNA was prepared
in situ in the agarose. Generation of the library closely
followed the cloning approach developed in Pieter de Jong’s
laboratory (Osoegawa
et al., 1998). DNA fragments from the appropriate size
fraction were cloned into the pBAC-VM4 vector (GenBank no.
pending). The ligation products were then transformed into
DH10B (T1 resistant) electro-competent cells (Invitrogen).
The library has been arrayed into 384-well microtiter dishes.
This work was funded by NIH grant HG02526-01 (Chris T. Amemiya,
VMRC).
Please note: The following data is provisional pending further
characterization.
| Segment |
Cloning Vector |
DNA |
Restriction enzyme |
Plate Number |
Total Plates |
Empty Wells |
Empty Wells (%) |
| 1 |
pBAC-VM4 |
Brain |
EcoRI |
1-402 |
402 |
0 |
0 |
| Segment |
Ghost clones (including vector artifacts) |
Recombinant clones |
Insert Size (Average) |
Redundancy (Genome Size: 2300 Mb) |
| 1 |
5% |
95% |
165 kb |
>= 10X |
|
