| CHORI-1224
Honey Bee Fosmid Library |
 |
The CHORI-1224 library has been constructed by Chung-Li Shu
and Kazutoyo Osoegawa
in Pieter de Jong's laboratory, BACPAC Resources, Children's
Hospital Oakland Research Institute. Agarose-embedded genomic
DNA was prepared from Apis mellifera DH4 strain by Dr. Stephen
Richards (Human Genome Sequencing Center Baylor College of
Medicine). To create fosmid clones, genomic DNA was sheared
by passing through a Hamilton syringe, then end-polished by
T4 DNA polymerase treatment. The end-repaired DNA was ligated
with pEpiFOS5 vector (Epicenter) and the ligation products
were incubated with "Gigapack III Gold" (Stratagene)
packaging extracts. The lambda-packaged DNA was transfected
into the DH5?MCR strain. Library construction was supported
by a sub-contract from a grant awarded to Richard Gibbs (Human
Genome Sequencing Center Baylor College of Medicine). The
collaborative effort has been organized by Daniel Weaver (President,
Bee Weaver Apiaries, Inc.).
| Cloning Vector |
DNA |
Restriction enzyme |
Plate Number |
Total Plates |
Empty Wells |
Empty Wells (%) |
Recombinant clones |
Average Insert Size |
Redundancy (Genome Size: 280 Mb) |
| pEpiFOS5 |
DH4 |
Sheared |
97-192 |
96 |
236 |
1.3 |
Approx. 36,630 |
36 kbp |
4.7 |
click here for a legend of
the previous tables.
Data on the CHORI-1224 clone average insert size has been
determined by Pulsed Field Gel Electrophoresis. Clone size
distribution has been plotted
graphically.
All library copies and individual clones should be maintained
using the recommended nomenclature guidelines as posted on
the NCBI
web site.
When the scientific results obtained from use of the CHORI-1224
library are published or otherwise disseminated, the scientific
creators and the origin of the BAC library should be acknowledged.
A recommended reference will be posted on this page as soon
as it is available. Otherwise, please contact Pieter
de Jong. This is important to permit us to measure the
impact of our scientific contributions and will facilitate
continued funding of similar resources.
With respect to the scheduling of shipments of arrayed library
copies, we would like to make you aware of our policy for
array distribution.
Please visit the ordering information page if you want to
place an order.
Reference: The Honeybee Genome Sequencing Consortium (2006) Insights into social insects from the genome of the honeybee Apis mellifera. Nature 443: 931-49.
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