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BAC Library Construction

 Home > Resources > Libraries
  CHORI-1224 Honey Bee Fosmid Library   

The CHORI-1224 library has been constructed by Chung-Li Shu and Kazutoyo Osoegawa in Pieter de Jong's laboratory, BACPAC Resources, Children's Hospital Oakland Research Institute. Agarose-embedded genomic DNA was prepared from Apis mellifera DH4 strain by Dr. Stephen Richards (Human Genome Sequencing Center Baylor College of Medicine). To create fosmid clones, genomic DNA was sheared by passing through a Hamilton syringe, then end-polished by T4 DNA polymerase treatment. The end-repaired DNA was ligated with pEpiFOS5 vector (Epicenter) and the ligation products were incubated with "Gigapack III Gold" (Stratagene) packaging extracts. The lambda-packaged DNA was transfected into the DH5?MCR strain. Library construction was supported by a sub-contract from a grant awarded to Richard Gibbs (Human Genome Sequencing Center Baylor College of Medicine). The collaborative effort has been organized by Daniel Weaver (President, Bee Weaver Apiaries, Inc.).

Cloning Vector DNA Restriction enzyme Plate Number Total Plates Empty Wells Empty Wells (%) Recombinant clones Average Insert Size Redundancy (Genome Size: 280 Mb)
pEpiFOS5 DH4 Sheared 97-192 96 236 1.3 Approx. 36,630 36 kbp 4.7

click here for a legend of the previous tables.
Data on the CHORI-1224 clone average insert size has been determined by Pulsed Field Gel Electrophoresis. Clone size distribution has been plotted graphically.
All library copies and individual clones should be maintained using the recommended nomenclature guidelines as posted on the NCBI web site.
When the scientific results obtained from use of the CHORI-1224 library are published or otherwise disseminated, the scientific creators and the origin of the BAC library should be acknowledged. A recommended reference will be posted on this page as soon as it is available. Otherwise, please contact Pieter de Jong. This is important to permit us to measure the impact of our scientific contributions and will facilitate continued funding of similar resources.

With respect to the scheduling of shipments of arrayed library copies, we would like to make you aware of our policy for array distribution.

Please visit the ordering information page if you want to place an order.



Reference: The Honeybee Genome Sequencing Consortium (2006) Insights into social insects from the genome of the honeybee Apis mellifera. Nature 443: 931-49.





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