Honey bee BAC Library
The CHORI-224 library
has been constructed by Chung-Li Shu and Kazutoyo
Osoegawa in Pieter de Jong's laboratory, BACPAC Resources,
Children's Hospital Oakland Research Institute. Agarose-embedded
genomic DNA was prepared from Apis mellifera DH4 strain by
Dr. Stephen Richards (Human Genome Sequencing Center Baylor
College of Medicine). To create the BAC clones, genomic DNA
was partially digested with a combination of EcoRI and EcoRI
Methylase. The restriction fragments were size-selected using
pulsed-field gel electrophoresis and then ligated into the
pTARBAC2.1 vector between the EcoRI sites. The ligation products
were transformed into DH10B (T1 phage-resistant) electro-competent
cells (Invitrogen). The library has been arrayed into 384-well
microtiter dishes and has also been gridded onto a 22x22cm
nylon high-density filter for screening by probe hybridization.
Each hybridization membrane represents about 18,000 distinct
BAC clones, each represented in duplicate. Library construction
was supported by a sub-contract from a grant awarded to Dr.
J. Spencer Johnston (Department of Entomology, Texas A&M
University). The collaborative effort has been organized by
Daniel Weaver (President, Bee Weaver Apiaries, Inc.).
data for CHORI-224 Honey bee BAC Library:
||Empty Wells (%)
|Non-insert clones (%)
||Non-Recombinant Clones (pUC)
||Average Insert Size
||Redundancy (Genome Size: 280 Mb)
Data on the CHORI-224
clone average insert size has been determined by Pulsed
Field Gel Electrophoresis. Clone size distribution has been
plotted graphically. While analyzing clones using pulse-field
electrophoresis to determine the average insert size, non-insert
clones containing a small deleted vector fragment consistent
with sucrose resistance were observed. Further in depth characterization
of the library is on going in our lab and data will be updated
on our web page periodically.
Please direct questions
concerning this library to either Pieter
J. de Jong or Kazutoyo
| Ordering &
The library is available
in several formats. Individual clones, and high-density hybridization
filters are obtainable. For ordering and shipping
details, please view the ordering
and pricing information page.
Academic and commercial
users interested in a copy of the BAC library should contact
Pieter J. de Jong ( email@example.com
), fax: (510) 450-7924).
Note: We are aware that about 30% of unique probes from honeybee fail to find corresponding sequences in the BAC library. This relates to the genomic composition of the honeybee genome. Please be aware of the risk that any positive clones may not be found even the library is screened with high-density filters using probes with good technical skills. It is user's responsibity to make a judgement whether or not the library should be screened. We will not be able to make any refund even the user fail to find any positive clones.
Reference: The Honeybee Genome Sequencing Consortium (2006)
Insights into social insects from the genome of the honeybee Apis mellifera. Nature 443: 931-49.