| CHORI-1106
Brugia malayi Fosmid Library |
 |
The CHORI-1106 (fosmid) library has been constructed by Chung-Li
Shu and Kazutoyo Osoegawa
in Pieter de Jong's laboratory, BACPAC Resources, Children's
Hospital Oakland Research Institute. Agarose-embedded genomic
DNA was prepared from Brugia malayi (TRS strain) by Dr. Jeremy
Foster (New England Boilabs). To create fosmid clones, genomic
DNA was sheared by passing through a Hamilton syringe. The
sheared fragments were size-purified using pulsed-field gel
electrophoresis then end-polished by T4 DNA polymerase treatment.
The end-repaired DNA was ligated with pEpiFOS5 vector (Epicenter)
and the ligation products were incubated with "Gigapack
III Gold" (Stratagene) packaging extracts. The lambda-packaged
DNA was transfected into the DH5?MCR strain. The CHORI-1106
library is comprised of 9,000 clones (plates 25-48) with an
average insert size of 43 kb. The total of 9,000 clones from
the library provides ~3.5X haploid genome coverage. The arrayed
clones have also been gridded together with CHORI-106
BAC library onto a 22x22cm nylon
high-density filter for screening by probe hybridization.
Each hybridization membrane represents about 9,000 distinct
BAC and 9,000 fosmid clones, each represented in duplicate.
Library construction was supported by a sub-contract from
a grant (NIH/NIAID U01-AI50903-02) awarded to Dr. Elodie Ghedin
at The Institute for Genomic Research (TIGR).
| Cloning Vector |
DNA |
Restriction enzyme |
Plate Number |
Total Plates |
Empty Wells |
Empty Wells (%) |
Recombinant clones |
Average Insert Size |
Redundancy (Genome Size:
110 Mb) |
| pEpiFOS5 |
TRS |
Sheared |
25-48 |
24 |
158 |
1.7 |
Approx. 9,060 |
43 kbp |
3.5 |
click here for a legend of
the previous tables.
Data on the CHORI-1106
clone average insert size has been determined by Pulsed
Field Gel Electrophoresis.
The library is available as library plates, individual clones,
and high-density hybridization sets (see Filter
Availability). The library is available for academic users
by reimbursement of duplication costs (labor & materials),
resulting in a price of $20 per 384-well dish. Dishes can
only be obtained per BAC and fosmid libraries (CHORI-106 and
CHORI-1106: $960) or per fosmid library ($480).Individual clones identified after screening can be obtained as stab cultures
at a cost of $25 per clone in addition to a $35 set-up charge per shipment. Shipping will be done by Federal Express and charged to the recipients account
number.
With respect to the scheduling of shipments of arrayed library copies, we would
like to make you aware of our policy for
array distribution.
Please visit the ordering information
page if you want to place an order.
Academic and commercial users interested in a copy of the BAC library should
contact Pieter J. de Jong (pdejong@chori.org, fax: (510) 450-7924). For hybridization
membranes, please contact BACPAC Resources (BACPACorders@chori.org).
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