Site Search by Google

Resources

Libraries
Human "32k" BAC Re-Array
GENSAT Collection
Vectors
Filters
Filter Interpreter Application
Mapped Clones
Non-Recombinants


Procedures
Online Mapping Resources
Download Page
Questions and Answers

Research

BAC Library Construction
ALS
Publications

 
 Home > Resources > Libraries
  CHORI-251 Chimpanzee BAC library

  

The CHORI-251 Chimpanzee BAC library has been constructed at the Children’s Hospital Oakland Research Institute, BACPAC Resources, by Dr. Baoli Zhu using the cloning techniques developed in our laboratory (Osoegawa et al., 1998). DNA was isolated from white blood cells obtained from a male chimpanzee (Pan troglodytes, "Clint", Yerkes #C0471; birthdate: 6-6-80). Agarose embedded high molecular weight DNA was partially digested with a combination of EcoRI restriction enzyme and EcoRI Methylase. Size fractionated DNA was cloned into the pTARBAC2.1 vector between the EcoRI sites. The ligation products were transformed into DH10B (T1 resistant) electrocompetent cells (BRL Life Technologies). The library has been arrayed into 384-well microtiter dishes and also gridded onto 12 22x22cm nylon high-density filter for screening by probe hybridization. Each hybridization membrane represents over 18,000 distinct rat BAC clones, stamped in duplicate. BAC library construction was funded by a grant from NIH (#HG01165-06).

Provisional Data for Chimpanzee BAC library:

Segment
DNA
Restriction Enzyme
Plate Numbers
Total Plates
Empty Wells
Empty Wells (%)
1
pTARBAC2.1
Adult Male Blood
EcoRI
1-288
288
1,630
1.5
2
pTARBAC2.1
Adult Male Blood
EcoRI
289-576
288
1,399
1.3
Total Library
 
 
 
1-576
576
3,029
1.4

 

Segment
Non-insert Clones (%)
Non-insert clones
Recombinant Clones
Insert size (Average)
Redundancy (Genome size:
3.3 KMb)
1
3.6
Approx. 3,923
552
Approx. 104,487
164 Kbp
5.2
2
6.0
Approx. 6,552
653
Approx. 101,988
176 Kbp
5.4
Total Library
4.6
Approx. 10,475
1,205
Approx 206,475
170 Kbp
10.6

click here for a legend of the previous tables.

The CHORI-251 clone average insert size has been determined by Pulsed Field Gel Electrophoresis. Clone size distribution has been plotted graphically.

The library is available as library plates, individual clones, and high-density hybridization sets (see Filter Availability)

Individual clones identified after screening can be obtained as stab cultures at a cost of $80 per clone in addition to a $35 set-up charge per shipment. Shipping will be done by Federal Express and charged to the recipients account number.

With respect to the scheduling of shipments of arrayed library copies, we would like to make you aware of our policy for array distribution.

Please visit the ordering information page if you want to place an order.

Academic and commercial users interested in a copy of the BAC library should contact Pieter J. de Jong (pdejong@chori.org, fax: (510) 450-7924). For hybridization membranes, please contact BACPAC Resources (BACPACorders@chori.org).

 


 

 

 

 

For questions related to the site, please contact webmaster.
The use of this website is subject to the terms of use.