Recommendations
on how to handle
the clones you receive |
 |
The clones you receive from us are shipped as bacterial LB
agar stab culture.The DH10 E.coli host that is harboring the
plasmid contains a DNA insert that has been placed into LB
agar (containing 12.5ug/ml chloramphenicol for BAC clones and
25 ug/ml kanamycin for PAC clones). This culture has a finite
life at 4°C. We recommend that you streak this culture
to single colonies on a LB agar plate with an antibiotic and
prepare a glycerol stock of the clones for long term storage
at -85°C. While our libraries have little well to well
contamination it would be wise, before initiating any experiments,
to check 3-5 colonies on CHEF gel or PCR. This to make sure
that the clone stock that we sent you is pure.
|
