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 Home > Products > Clones 
 Individual clone information     

 

  Availability

Note: All clones have names, but most names cannot be found on our web site. The clone names are usually found by the end user as listed in various public databases and publications. The clones names mostly follow the NCBI clone nomenclature rules. The clones lack catalog numbers. Costs of clones fall in a number of simple classes as defined on our ordering instructions page

All of this may appear confusing: for instance, how does one know if BACPAC Resources Center (BPRC) has a particular clone when the clones are not listed?

BPRC does not list clones, for two reasons: 1)most clones have not been characterized and lack specific data. 2)all clones are part of libraries and all clones from a particular library share common charactestics.  Hence, to find out if BPRC has a particular clone, one needs either use "Automatic Clone Validation" or else find out if the clone is compatible with the range of clone names for a corresponding clone library. Typically (although not always), clone names are derived from the library name. We use the NCBI-recommended clone nomenclature & library nomenclature.  For instance clone "RP11-103B2" indicates a clone in BAC library "RP11", a.k.a "RPCI-11". More precisely, it is a clone found in microtiter dish "103" at the intersection of row "B" and column "2".  To find out if BPRC has this clone, one needs to find the library-specific web page for the "RP11" BAC library and check if a microtiter dish "103" exists and if the row/column intersection is compatible with a 384-well dish. To find the BAC library, please visit our library browser by following the "Libraries" link on the left sidebar. The "RP11" library web page contains additional information and links, for instance with respect to the vector graphics & sequence, the method of cloning and publications.

  Automatic Clone Validation

One can check the "likely" availability of the clone also in an automatic way without going through the library pages. Please paste the clone name or a series of clone names in the clone verification window on the "Online Clone Ordering" page [This does not require any commitment to proceed with the ordering process]. Clones which cannot be confirmed, can nevertheless be present in our repository. For instance, the clone name might have been misspelled or be in a non-standard format. Please check the NCBI nomenclature, and convert the clone name to a compatible format, and then try again. If the clone is not confirmed and the corresponding library is not listed on our library browser, then the clone is most likely not in our repository.   Another place to check is the NCBI Clone registry which has a listing of all human and mouse BAC clones but is not complete with respect to many other libraries. If the Clone Registry has the clone information, it most likely will indicate the distributor where the clone can be obtained.

Clones from libraries with an "RP" or "RPCI" name are unavailable to commercial users.

Clones are available from any of our libraries as LB STAB cultures. For more information on how to order, please view the ordering and pricing information page.

  Clone Mapping Information

Information about the clones you order can be obtained from online mapping resources such as the National Center for Biotechnology Information (NCBI), the Human Genome Browser at the University of California Santa Cruz or the ensembl server. The following table contains a list of mapping resources links to websites, which are commonly used for finding BAC clones related to a certain gene, mRNA or genome section of your interest.

-National Center for Biotechnology Information
-UCSC Human Genome browser
-Ensembl
-SRS Server
-The Human BAC Clone Resource for Identification of Cancer Aberrations
-Mouse Mapping Consortium: RPCI-23 Mouse BAC Mapping
-FruitFly.org for drosophila cDNA libraries

 

 

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