clones have names, but most names cannot be found on our web
site. The clone names are usually found by the end user as
listed in various public databases and publications. The clones
names mostly follow the NCBI clone nomenclature rules. The
clones lack catalog numbers. Costs of clones fall in a number
of simple classes as defined on our ordering
of this may appear confusing: for instance, how does one know
if BACPAC Resources Center (BPRC) has a particular clone when
the clones are not listed?
BPRC does not
list clones, for two reasons: 1)most clones have not been
characterized and lack specific data. 2)all clones are part
of libraries and all clones from a particular library share
common charactestics. Hence, to find out if BPRC has
a particular clone, one needs either use "Automatic Clone
Validation" or else find out if the clone is compatible
with the range of clone names for a corresponding clone library.
Typically (although not always), clone names are derived from
the library name. We use the NCBI-recommended clone
nomenclature & library
nomenclature. For instance clone "RP11-103B2"
indicates a clone in BAC library "RP11", a.k.a "RPCI-11".
More precisely, it is a clone found in microtiter dish "103"
at the intersection of row "B" and column "2".
To find out if BPRC has this clone, one needs to find the
library-specific web page for the "RP11" BAC library
and check if a microtiter dish "103" exists and
if the row/column intersection is compatible with a 384-well
dish. To find the BAC library, please visit our library
browser by following the "Libraries" link on
the left sidebar. The "RP11"
library web page contains additional information and links,
for instance with respect to the vector graphics & sequence,
the method of cloning and publications.
Automatic Clone Validation
One can check the
"likely" availability of the clone also in an automatic
way without going through the library pages. Please paste
the clone name or a series of clone names in the clone verification
window on the "Online Clone
Ordering" page [This does not require any commitment
to proceed with the ordering process]. Clones which cannot
be confirmed, can nevertheless be present in our repository.
For instance, the clone name might have been misspelled or
be in a non-standard format. Please check the NCBI nomenclature,
and convert the clone name to a compatible format, and then
try again. If the clone is not confirmed and the corresponding
library is not listed on our library
browser, then the clone is most likely not in our repository.
Another place to check is the NCBI
Clone registry which has a listing of all human and mouse
BAC clones but is not complete with respect to many other
libraries. If the Clone Registry has the clone information,
it most likely will indicate the distributor where the clone
can be obtained.
Clones from libraries
with an "RP" or "RPCI" name are unavailable to commercial
Clones are available
from any of our libraries as LB STAB cultures. For more information
on how to order, please view the ordering
and pricing information page.
Clone Mapping Information
the clones you order can be obtained from online mapping resources
such as the National Center for Biotechnology Information
(NCBI), the Human Genome Browser at the University of California
Santa Cruz or the ensembl server. The following table contains
a list of mapping resources links to websites, which are commonly
used for finding BAC clones related to a certain gene, mRNA
or genome section of your interest.
Center for Biotechnology Information
Human Genome browser
Human BAC Clone Resource for Identification of Cancer Aberrations
Mapping Consortium: RPCI-23 Mouse BAC Mapping
for drosophila cDNA libraries