LOCUS pBACGK1.1 11300 bp DNA circular 11-MAR-2004 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT VNTDATE|298720231| COMMENT VNTDBDATE|332787232| COMMENT VNTNAME|pBACGK1.1| COMMENT VNTAUTHORNAME|Kazutoyo Osoegawa| COMMENT VNTAUTHOREML|kosoegawa@chori.org| COMMENT VNTOAUTHORNAME|UNKNOWN| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Bacteria FEATURES Location/Qualifiers CDS 2934..4352 /vntifkey="4" /label=SacB misc_feature 1..2810 /vntifkey="21" /label=pUC-Link CDS complement(6348..7523) /vntifkey="4" /label=parA CDS complement(5377..6348) /vntifkey="4" /label=parB CDS complement(8102..8857) /vntifkey="4" /label=repE misc_feature 11068..11104 /vntifkey="21" /label=PI-SceI misc_feature 2817..2834 /vntifkey="21" /label=SP6 misc_feature 11277..11299 /vntifkey="21" /label=T7 promoter 11228..11268 /vntifkey="30" /label=SacB\gene\promoter CDS 10197..10856 /vntifkey="4" /label=CM(R) misc_feature 4787..5304 /vntifkey="21" /label=parC rep_origin 9196..9251 /vntifkey="33" /label=oriS misc_feature complement(9631..9978) /vntifkey="21" /label=redF misc_feature 11105..11129 /vntifkey="21" /label=attB1 misc_feature complement(2855..2879) /vntifkey="21" /label=attB2 BASE COUNT 3196 a 2725 c 2636 g 2743 t ORIGIN 1 gatccgcgga attcgagctc acgcgtactg atgcatgatc cgggtttaaa cccagtactc 61 tagatcctct agagtcgacc tgcaggcatg caagcttggc gtaatcatgg tcatagctgt 121 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 181 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 241 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 301 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 361 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 421 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 481 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 541 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 601 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 661 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta 721 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 781 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 841 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 901 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat 961 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 1021 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 1081 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 1141 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 1201 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 1261 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 1321 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 1381 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 1441 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 1501 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 1561 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 1621 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 1681 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 1741 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 1801 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 1861 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 1921 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 1981 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 2041 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 2101 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 2161 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 2221 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta 2281 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt 2341 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc 2401 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt 2461 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc 2521 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat 2581 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc 2641 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt 2701 cacgacgttg taaaacgacg gccagtgaat tcgagctcgg tacccgggga tctagagtac 2761 tggttgaaac ccggatcatg catcagtacg cgtgagctcg aattccgcgg atccttctat 2821 agtgtcacct aaatgtcgac ggccaggcgg ccgcacccag ctttcttgta caaagtggtg 2881 aggatcgaaa cggcagatcg caaaaaacag tacatacaga aggagacatg aacatgaaca 2941 tcaaaaaaat tgtaaaacaa gccacagttc tgacttttac gactgcactt ctggcaggag 3001 gagcgactca agccttcgcg aaagaaaata accaaaaagc atacaaagaa acgtacggcg 3061 tctctcatat tacacgccat gatatgctgc agatccctaa acagcagcaa aacgaaaaat 3121 accaagtgcc tcaattcgat caatcaacga ttaaaaatat tgagtctgca aaaggacttg 3181 atgtgtggga cagctggccg ctgcaaaacg ctgacggaac agtagctgaa tacaacggct 3241 atcacgttgt gtttgctctt gcgggaagcc cgaaagacgc tgatgacaca tcaatctaca 3301 tgttttatca aaaggtcggc gacaactcaa tcgacagctg gaaaaacgcg ggccgtgtct 3361 ttaaagacag cgataagttc gacgccaacg atccgatcct gaaagatcag acgcaagaat 3421 ggtccggttc tgcaaccttt acatctgacg gaaaaatccg tttattctac actgactatt 3481 ccggtaaaca ttacggcaaa caaagcctga caacagcgca ggtaaatgtg tcaaaatctg 3541 atgacacact caaaatcaac ggagtggaag atcacaaaac gatttttgac ggagacggaa 3601 aaacatatca gaacgttcag cagtttatcg atgaaggcaa ttatacatcc ggcgacaacc 3661 atacgctgag agaccctcac tacgttgaag acaaaggcca taaatacctt gtattcgaag 3721 ccaacacggg aacagaaaac ggataccaag gcgaagaatc tttatttaac aaagcgtact 3781 acggcggcgg cacgaacttc ttccgtaaag aaagccagaa gcttcagcag agcgctaaaa 3841 aacgcgatgc tgagttagcg aacggcgccc tcggtatcat agagttaaat aatgattaca 3901 cattgaaaaa agtaatgaag ccgctgatca cttcaaacac ggtaactgat gaaatcgagc 3961 gcgcgaatgt tttcaaaatg aacggcaaat ggtacttgtt cactgattca cgcggttcaa 4021 aaatgacgat cgatggtatt aactcaaacg atatttacat gcttggttat gtatcaaact 4081 ctttaaccgg cccttacaag ccgctgaaca aaacagggct tgtgctgcaa atgggtcttg 4141 atccaaacga tgtgacattc acttactctc acttcgcagt gccgcaagcc aaaggcaaca 4201 atgtggttat cacaagctac atgacaaaca gaggcttctt cgaggataaa aaggcaacat 4261 ttgcgccaag cttcttaatg aacatcaaag gcaataaaac atccgttgtc aaaaacagca 4321 tcctggagca aggacagctg acagtcaact aataacagca aaaagaaaat gccgatactt 4381 cattggcatt ttcttttatt tctcaacaag atggtgaatt gactagtggg tagatccaca 4441 ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca 4501 ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt 4561 gcatcaacgc atatagcgct agctcggaat ggacgatatc ccgcgacagc gacacacttg 4621 cgttaacgtg ccggcacggc ctgggtaacc aggtattttg tccacataac cgtgcgcaaa 4681 atgttgtgga taagcaggac acagcagcaa tccacagcag gcatacaacc gcacaccgag 4741 gttactccgt tctacaggtt acgacgacat gtcaatactt gcccttgaca ggcattgatg 4801 gaatcgtagt ctcacgctga tagtctgatc gacaatacaa gtgggaccgt ggtcccagac 4861 cgataatcag accgacaaca cgagtgggat cgtggtccca gactaataat cagaccgacg 4921 atacgagtgg gaccgtggtc ccagactaat aatcagaccg acgatacgag tgggaccgtg 4981 gttccagact aataatcaga ccgacgatac gagtgggacc gtggtcccag actaataatc 5041 agaccgacga tacgagtggg accatggtcc cagactaata atcagaccga cgatacgagt 5101 gggaccgtgg tcccagtctg attatcagac cgacgatacg agtgggaccg tggtcccaga 5161 ctaataatca gaccgacgat acgagtggga ccgtggtccc agactaataa tcagaccgac 5221 gatacgagtg ggaccgtggt cccagtctga ttatcagacc gacgatacaa gtggaacagt 5281 gggcccagag agaatattca ggccagttat gctttctggc ctgtaacaaa ggacattaag 5341 taaagacaga taaacgtaga ctaaaacgtg gtcgcatcag ggtgctggct tttcaagttc 5401 cttaagaatg gcctcaattt tctctataca ctcagttgga acacgagacc tgtccaggtt 5461 aagcaccatt ttatcgccct tatacaatac tgtcgctcca ggagcaaact gatgtcgtga 5521 gcttaaacta gttcttgatg cagatgacgt tttaagcaca gaagttaaaa gagtgataac 5581 ttcttcagct tcaaatatca ccccagcttt tttctgctca tgaaggttag atgcctgctg 5641 cttaagtaat tcctctttat ctgtaaaggc tttttgaagt gcatcacctg accgggcaga 5701 tagttcaccg gggtgagaaa aaagagcaac aactgattta ggcaatttgg cggtgttgat 5761 acagcgggta ataatcttac gtgaaatatt ttccgcatca gccagcgcag aaatatttcc 5821 agcaaattca ttctgcaatc ggcttgcata acgctgacca cgttcataag cacttgttgg 5881 gcgataatcg ttacccaatc tggataatgc agccatctgc tcatcatcca gctcgccaac 5941 cagaacacga taatcacttt cggtaagtgc agcagcttta cgacggcgac tcccatcggc 6001 aatttctatg acaccagata ctcttcgacc gaacgccggt gtctgttgac cagtcagtag 6061 aaaagaaggg atgagatcat ccagtgcgtc ctcagtaagc agctcctggt cacgttcatt 6121 acctgaccat acccgagagg tcttctcaac actatcaccc cggagcactt caagagtaaa 6181 cttcacatcc cgaccacata caggcaaagt aatggcatta ccgcgagcca ttactcctac 6241 gcgcgcaatt aacgaatcca ccatcggggc agctggtgtc gataacgaag tatcttcaac 6301 cggttgagta ttgagcgtat gttttggaat aacaggcgca cgcttcatta tctaatctcc 6361 cagcgtggtt taatcagacg atcgaaaatt tcattgcaga caggttccca aatagaaaga 6421 gcatttctcc aggcaccagt tgaagagcgt tgatcaatgg cctgttcaaa aacagttctc 6481 atccggatct gacctttacc aacttcatcc gtttcacgta caacattttt tagaaccatg 6541 cttccccagg catcccgaat ttgctcctcc atccacgggg actgagagcc attactattg 6601 ctgtatttgg taagcaaaat acgtacatca ggctcgaacc ctttaagatc aacgttcttg 6661 agcagatcac gaagcatatc gaaaaactgc agtgcggagg tgtagtcaaa caactcagca 6721 ggcgtgggaa caatcagcac atcagcagca catacgacat taatcgtgcc gatacccagg 6781 ttaggcgcgc tgtcaataac tatgacatca tagtcatgag caacagtttc aatggccagt 6841 cggagcatca ggtgtggatc ggtgggcagt ttaccttcat caaatttgcc cattaactca 6901 gtttcaatac ggtgcagagc cagacaggaa ggaataatgt caagccccgg ccagcaagtg 6961 ggctttattg cataagtgac atcgtccttt tccccaagat agaaaggcag gagagtgtct 7021 tctgcatgaa tatgaagatc tggtacccat ccgtgataca ttgaggctgt tccctggggg 7081 tcgttacctt ccacgagcaa aacacgtagc cccttcagag ccagatcctg agcaagatga 7141 acagaaactg aggttttgta aacgccacct ttatgggcag caaccccgat caccggtgga 7201 aatacgtctt cagcacgtcg caatcgcgta ccaaacacat cacgcatatg attaatttgt 7261 tcaattgtat aaccaacacg ttgctcaacc cgtcctcgaa tttccatatc cgggtgcggt 7321 agtcgccctg ctttctcggc atctctgata gcctgagaag aaaccccaac taaatccgct 7381 gcttcaccta ttctccagcg ccgggttatt ttcctcgctt ccgggctgtc atcattaaac 7441 tgtgcaatgg cgatagcctt cgtcatttca tgaccagcgt ttatgcactg gttaagtgtt 7501 tccatgagtt tcattctgaa catcctttaa tcattgcttt gcgttttttt attaaatctt 7561 gcaatttact gcaaagcaac aacaaaatcg caaagtcatc aaaaaaccgc aaagttgttt 7621 aaaataagag caacactaca aaaggagata agaagagcac atacctcagt cacttattat 7681 cactagcgct cgccgcagcc gtgtaaccga gcatagcgag cgaactggcg aggaagcaaa 7741 gaagaactgt tctgtcagat agctcttacg ctcagcgcaa gaagaaatat ccaccgtggg 7801 aaaaactcca ggtagaggta cacacgcgga tagccaattc agagtaataa actgtgataa 7861 tcaaccctca tcaatgatga cgaactaacc cccgatatca ggtcacatga cgaagggaaa 7921 gagaaggaaa tcaactgtga caaactgccc tcaaatttgg cttccttaaa aattacagtt 7981 caaaaagtat gagaaaatcc atgcaggctg aaggaaacag caaaactgtg acaaattacc 8041 ctcagtaggt cagaacaaat gtgacgaacc accctcaaat ctgtgacaga taaccctcag 8101 actatcctgt cgtcatggaa gtgatatcgc ggaaggaaaa tacgatatga gtcgtctggc 8161 ggcctttctt tttctcaatg tatgagaggc gcattggagt tctgctgttg atctcattaa 8221 cacagacctg caggaagcgg cggcggaagt caggcatacg ctggtaactt tgaggcagct 8281 ggtaacgctc tatgatccag tcgattttca gagagacgat gcctgagcca tccggcttac 8341 gatactgaca cagggattcg tataaacgca tggcatacgg attggtgatt tcttttgttt 8401 cactaagccg aaactgcgta aaccggttct gtaacccgat aaagaaggga atgagatatg 8461 ggttgatatg tacactgtaa agccctctgg atggactgtg cgcacgtttg ataaaccaag 8521 gaaaagattc atagcctttt tcatcgccgg catcctcttc agggcgataa aaaaccactt 8581 ccttccccgc gaaactcttc aatgcctgcc gtatatcctt actggcttcc gcagaggtca 8641 atccgaatat ttcagcatat ttagcaacat ggatctcgca gataccgtca tgttcctgta 8701 gggtgccatc agattttctg atctggtcaa cgaacagata cagcatacgt ttttgatccc 8761 gggagagact atatgccgcc tcagtgaggt cgtttgactg gacgattcgc gggctatttt 8821 tacgtttctt gtgattgata accgctgttt ccgccatgac agatccatgt gaagtgtgac 8881 aagtttttag attgtcacac taaataaaaa agagtcaata agcagggata actttgtgaa 8941 aaaacagctt cttctgaggg caatttgtca cagggttaag ggcaatttgt cacagacagg 9001 actgtcattt gagggtgatt tgtcacactg aaagggcaat ttgtcacaac accttctcta 9061 gaaccagcat ggataaaggc ctacaaggcg ctctaaaaaa gaagatctaa aaactataaa 9121 aaaaataatt ataaaaatat ccccgtggat aagtggataa ccccaaggga agttttttca 9181 ggcatcgtgt gtaagcagaa tatataagtg ctgttccctg gtgcttcctc gctcactcga 9241 gggcttcgcc ctgtcgctca actgcggcga gcactactgg ctgtaaaagg acagaccaca 9301 tcatggttct gtgttcatta ggttgttctg tccattgctg acataatccg ctccacttca 9361 acgtaacacc gcacgaagat ttctattgtt cctgaaggca tattcaaatc gttttcgtta 9421 ccgcttgcag gcatcatgac agaacactac ttcctataaa cgctacacag gctcctgaga 9481 ttaataatgc ggatctctac gataatggga gattttcccg actgtttcgt tcgcttctca 9541 gtggataaca gccagcttct ctgtttaaca gacaaaaaca gcatatccac tcagttccac 9601 atttccatat aaaggccaag gcatttattc tcaggataat tgtttcagca tcgcaaccgc 9661 atcagactcc ggcatcgcaa actgcacccg gtgccgggca gccacatcca gcgcaaaaac 9721 cttcgtgtag acttccgttg aactgatgga cttatgtccc atcaggcttt gcagaacttt 9781 cagcggtata ccggcataca gcatgtgcat cgcataggaa tggcggaacg tatgtggtgt 9841 gaccggaaca gagaacgtca caccgtcagc agcagcggcg gcaaccgcct ccccaatcca 9901 ggtcctgacc gttctgtccg tcacttccca gatccgcgct ttctctgtcc ttcctgtgcg 9961 acggttacgc cgctccatga gcttatcgcg aataaatacc tgtgacggaa gatcacttcg 10021 cagaataaat aaatcctggt gtccctgttg ataccgggaa gccctgggcc aacttttggc 10081 gaaaatgaga cgttgatcgg cacgtaagag gttccaactt tcaccataat gaaataagat 10141 cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg 10201 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt 10261 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta 10321 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca 10381 ttcttgcccg cctgatgaat gctcatccgg agttccgtat ggcaatgaaa gacggtgagc 10441 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt 10501 tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc 10561 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata 10621 tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca 10681 atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca 10741 aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg 10801 gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttt 10861 tttaaggcag ttattggtgc ccttaaacgc ctggttgcta cgcctgaata agtgataata 10921 agcggatgaa tggcagaaat tcgatgataa gctgtcaaac atgagaattg gtcgacggcg 10981 cgccaaagct tgcatgcctg cagccgcgta acctggcaaa atcggttacg gttgagtaat 11041 aaatggatgc cctgcgtaag cggggcacat ttcattacct ctttctccgc acccgacata 11101 gataacaagt ttgtacaaaa aagcaggctc tagtagactt aattaaggat cgatccggcg 11161 cgccaatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg 11221 gcatcggtcg agcttgacat tgtaggacta tattgctcta ataaatttgc ggccgctaat 11281 acgactcact atagggagag //