| Drosophila
Fosmid Libraries
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Genomic fosmid libraries were constructed
for the Berkeley Drosophila Genome Project by Andreas Gnirke
at Exelixis, Inc. (South San Francisco) from Drosophila stocks
obtained from various sources. D. erecta and D. pseudoobscura
stocks were obtained from the Drosophila species stock center,
which has relocated to Tuscon (http://stockcenter.arl.arizona.edu/).
The D. littoralis stock was obtained from the Umea, Sweden
stock center via Kevin White (Yale). The Umea stock center
is now closed. The D. willistoni stock was obtained from Jeffrey
Powell (Yale). The species identifications of the stocks were
confirmed by David Grimaldi (American Museum of Natural History,
NY). To make the libraries, genomic DNA was partially digested
with MboI, size-selected by pulsed-field-gel electrophoresis
and cloned in the BamHI site of the fosmid vector pFOS1 (U.-J.
Kim et al. (1992) Nucleic Acids Res. 20: 1083 -1085) in the
E. coli host strain XL1-Blue MR (Stratagene).
library species
stock information clone nomenclature
DERF01 D. erecta Tucson #14021-0224.0 DER36E01 (Plate Information)
DPSF01 D. pseudoobscura Tucson #14011-0121.4 DPS24A05 (Plate Information)
DVIF01 D. virilis* [Umea #S060 (Kevin White) DLI14B24] (Plate Information)
DWIF01 D. willistoni Guadeloupe (Jeffrey Powell) DWI04E31 (Plate Information)
* The DVIF01 library
was previously thought to be derived from D. littoralis and
was thus previously known as "DLIF01". Upon sequencing
of several clones, it became apparent that a strain mixup
might have occurred. Using appropiate fly genetics at the
Berkeley Drosophila Genome Project Sequencing Center, the
source of the library was unambiguously identified as D.virilis.
(Susan Celniker, personal communication)
Link
to information on pFOS1
A map of pFOS1 is available there:
Click on "protocols", "construction of BAC
libraries", and "pFOS1" to get a
map of the pFOS1 plasmid. To prepare the vector arms the plasmid
is
linearized at the AatII site, dephosphorylated and then digested
with BamHI.
Please note that the pUC-derived portion of the plasmid (between
the two cos
sites) is lost during in vitro packaging.
Vector
sequence information
Link
to Drosophila species stock center in Tucson, AZ
Individual clones identified after screening can be obtained as stab cultures at a cost of $80 per clone in addition to a $35 set-up charge per shipment.
Shipping will be done by Federal Express and charged to recipients
account number.
With respect to the scheduling of shipments of arrayed library copies, we would
like to make you aware of our policy for
array distribution.
Please visit the ordering information
page if you want to place an order.
Academic and commercial users interested in a copy of the BAC library should
contact Pieter J. de Jong (pdejong@chori.org, fax: (510) 450-7924). For hybridization
membranes, please contact BACPAC Resources (BACPACorders@chori.org).
Academic
and commercial users interested in a copy of the BAC library
should contact Pieter J. de Jong [email: pdejong@chori, fax:
(510) 540-7924].
With respect to the scheduling of shipments of arrayed library copies, we would
like to make you aware of our policy for
array distribution.
Please visit the ordering information
page if you want to place an order.
Academic and commercial users interested in a copy of the BAC library should
contact Pieter J. de Jong (pdejong@chori.org, fax: (510) 450-7924). For hybridization
membranes, please contact BACPAC Resources (BACPACorders@chori.org).
With respect to the scheduling of shipments of arrayed library copies, we would
like to make you aware of our policy for
array distribution.
Please visit the ordering information
page if you want to place an order.
Academic and commercial users interested in a copy of the BAC library should
contact Pieter J. de Jong (pdejong@chori.org, fax: (510) 450-7924). For hybridization
membranes, please contact BACPAC Resources (BACPACorders@chori.org).
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