| RPCI
- 21 Female (129S6/SvEvTac) Mouse PAC Library
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The RPCI-21 PAC Library has been
constructed in our laboratory by Kazutoyo
Osoegawa. PAC library construction was funded by grants
from the US Department of Energy (#DE-FG02-94ER61883) and
the National Human Genome Research Institute (NHGRI, NIH)
(#1R01RG01165-03). female 129S6/SvEvTac mouse spleen
genomic DNA (partially MboI digested) was cloned between the
Bam HI sites of the pPAC4 vector.
The ligation products were transformed into DH10B electrocompetent
cells (BRL Life technologies). The library has been
arrayed into 384 well microtiter dishes and then gridded onto
22x22cm nylon high density hybridization
filters for screening by probe hybridization. Each
hybridization membrane represents over 18,000 distinct mouse
PAC clones, stamped in duplicate.
The Total RPCI-21
Mouse PAC Library:
Clones Picked |
Empty Wells |
% Empty |
Non Recombinant
Clones |
Non Recombinant
Clones (%) |
Total Clones |
Average Insert Size |
384 Well Plate Numbers |
| 258048 |
6565 |
2.5 |
1312 |
0.5 |
250040 |
137 Kbp |
1-672 |
Segments |
Clones Picked |
Empty Wells |
Non Recombinant
(Total) |
Non Recombinant
Clones (%) |
% Empty |
Total Clones |
Average Insert Size |
384 Well Plate Numbers |
Gemonic Coverage |
1 |
129836 |
3868 |
650 |
0.5 |
3.0 |
125318 |
127 Kbp |
1-336 |
5.3X |
2 |
132473 |
2912 |
662 |
0.5 |
2.2 |
128899 |
147 Kbp |
337-672 |
6.3X |
click here
for a legend of the previous tables.
The average
insert size data has been determined by Pulsed field Gel
Electrophoresis.
Reference:
Osoegawa, K. et al. (2000) Bacterial Artificial Chromosome
Libraries for Mouse Sequencing and Functional Analysis.
Genome Research 10: 116-128
Reprints availiable upon request.
The library is
available for individual clone orders, distributed as agar-stab
cultures at a not-for profit cost. See our webpage "Ordering
& Pricing Information" for
the costs .
The PAC library has been gridded onto 22x22cm positively charged
nylon filters for hybridization screening purposes. Each filter
contains 36,864 colonies which represents 18,432 independent
clones spotted in duplicate in a 4x4 clone array. Clones can
be identified through screening by purchasing "high-density
colony" hybridization filters or by utilizing our "fee
for service" "Library Screening
Services" . For questions about clones and hybridization
membranes, please contact BACPAC Resources (BACPACorders@chori.org).
With respect to the scheduling of shipments of arrayed library copies, we would
like to make you aware of our policy for
array distribution.
Please visit the ordering information
page if you want to place an order.
Academic and commercial users interested in a copy of the BAC library should
contact Pieter J. de Jong (pdejong@chori.org, fax: (510) 450-7924). For hybridization
membranes, please contact BACPAC Resources (BACPACorders@chori.org).
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