Site Search by Google

Resources

Libraries
Human "32k" BAC Re-Array
GENSAT Collection
Vectors
Filters
Filter Interpreter Application
Mapped Clones
Non-Recombinants


Procedures
Online Mapping Resources
Download Page
Questions and Answers

Research

BAC Library Construction
ALS
Publications

 
 Home > Resources > Libraries
   CHORI-254: Squirrel Monkey (Saimiri boliviensis boliviensis) BAC Library   

Background information

The CHORI-254 Bolivian squirrel monkey (Saimiri boliviensis boliviensis, male) BAC library has been constructed at the Children's Hospital Oakland Research Institute, BACPAC Resources, by Doriana Misceo and Dr. Baoli Zhu using the cloning techniques developed in our laboratory (Osoegawa et al., 1998). The species and specimen information is Saimiri boliviensis boliviensis (Bolivian squirrel monkey), Male, Date of birth 6/16/1999, Specimen ID #3068. The specimen was provided by Dr. Christian R. Abee, The Squirrel Monkey Breeding and Research Resource (SMBRR - IPBRR), Department of Comparative Medicine, University of South Alabama, Mobile, AL. DNA was isolated from fresh kidney cells after preparing a cell suspension using a Dounce homogenizer with a tight pestle clearance: 0.001-0.003 inches. Agarose-embedded high molecular weight DNA was partially digested with a combination of EcoRI restriction enzyme and EcoRI methylase. Size fractionated DNA was ligated into the pTARBAC2.1 vector between the EcoRI sites. The ligation products were transformed into DH10B (T1 resistant) electrocompetent cells (Invitrogen). The library has been arrayed into 384-well microtiter dishes and also gridded onto 10 22x22cm nylon high-density filter for screening by probe hybridization. Each hybridization membrane represents over 18,000 distinct BAC clones, stamped in duplicate.

Segment

1

2

All

Vector

pTARBAC2.1

pTARBAC2.1

pTARBAC2.1

Restriction Enzyme

EcoRI/EcoRI Methylase

EcoRI/EcoRI Methylase

EcoRI/EcoRI Methylase

DNA Source

kidney

kidney

kidney

Plate Numbers

1-240

241-480

1-480

Plate Count

240

240

480

Empty Wells

2748 (2.98%)

1981 (2.15%)

4729 (2.57%)

Non-Recombinant Clones

N/A

N/A

N/A

Non-Insert Clones

N/A

N/A

N/A

Recombinant Clones

89412

90179

179591

Average Insert Size

N/A

N/A

N/A

Genomic Coverage

N/A

N/A

N/A



Click here for a legend of the previous tables.

The library is available as library plates, individual clones, and high-density hybridization sets (see Filter Availability)

Individual clones identified after screening can be obtained as stab cultures at a cost of $80 per clone in addition to a $35 set-up charge per shipment. Shipping will be done by Federal Express and charged to the recipients account number.


With respect to the scheduling of shipments of arrayed library copies, we would like to make you aware of our policy for array distribution.

Please visit the ordering information page if you want to place an order.

Academic and commercial users interested in a copy of the BAC library should contact Pieter J. de Jong (pdejong@chori.org, fax: (510) 450-7924). For hybridization membranes, please contact BACPAC Resources (BACPACorders@chori.org).

Content Posted: Sep 30th 2003

Content updated: December 1st 2003

 

For questions related to the site, please contact webmaster.
The use of this website is subject to the terms of use.