Site Search by Google

Resources

Libraries
Human "32k" BAC Re-Array
GENSAT Collection
Vectors
Filters
Filter Interpreter Application
Mapped Clones
Non-Recombinants


Procedures
Online Mapping Resources
Download Page
Questions and Answers

Research

BAC Library Construction
ALS
Publications

 
 Home > Resources > Libraries
   VMRC-11: Rattus norvegicus (F) (SS/JrHsd/Mcwi) BAC Library   

This rat (Rattus norvegicus) BAC library was constructed by Wendy Lippmann and Joshua Danke in Chris Amemiya’s laboratory at the Benaroya Research Institute at Virginia Mason (BRI). This library was designated VMRC-11. A freshly dissected brain tissue sample was obtained from a male rat (strain SS/JrHsd/Mcwi) from Drs. Joe Lazar and Howard Jacob (Medical College of Wisconsin). Nuclei were isolated using a Dounce homogenizer, embedded in Incert agarose; high molecular weight DNA was prepared in situ in agarose. Generation of the library followed the general cloning approach developed in Pieter de Jong’s laboratory (Osoegawa et al., 1998). EcoRI fragments from the appropriate size fraction were cloned into the EcoRI site of the pCCBAC vector (Epicentre Technologies). The ligation products were then transformed into DH10B (T1 resistant) electro-competent cells (Invitrogen). The library has been arrayed into 384-well microtiter dishes. Analysis of ~ 200 randomly selected clones from the library were used to assess the quality of the library. This work was funded by NIH grant HG02526 (Chris T. Amemiya, BRI).

Segment

1

All

Vector

pCC1BAC

Restriction Enzyme

N/A

N/A

DNA Source

N/A

N/A

Plate Numbers

1-528

1-528

Plate Count

528

528

Empty Wells

3.6 (0%)

N/A

Non-Recombinant Clones

N/A

N/A

Non-Insert Clones

N/A

N/A

Recombinant Clones

202748.4

0

Average Insert Size

N/A

N/A

Genomic Coverage

N/A

N/A

 

For questions related to the site, please contact webmaster.
The use of this website is subject to the terms of use.