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VMRC-17: Rat (F) (Rattus norvegicus,FHH/Eur) BAC Library

This rat (Rattus norvegicus) BAC library was constructed by Alicia Hill-Force and Joshua Danke in Chris Amemiya’s laboratory at the Benaroya Research Institute at Virginia Mason (BRI). This library was designated VMRC-17. A freshly dissected brain tissue sample was obtained from a male rat (strain FHH/EUR) from Drs. Joe Lazar and Howard Jacob (Medical College of Wisconsin). Nuclei were isolated using a Dounce homogenizer, embedded in Incert agarose; high molecular weight DNA was prepared in situ in the agarose. Generation of the library followed the general cloning approach developed in Pieter de Jong’s laboratory (Osoegawa et al., 1998). EcoRI fragments from the appropriate size fraction were cloned into the EcoRI site of the pCCBAC vector (Epicentre Technologies). The ligation products were then transformed into DH10B (T1 resistant) electro-competent cells (Invitrogen). The library has been arrayed into 384-well microtiter dishes. Analysis of ~ 200 randomly selected clones from the library were used to assess the quality of the library. This work was funded by NIH grant HG02526 (Chris T. Amemiya, BRI).