| RPCI
- 81 Male (Doberman Pinscher) Canine BAC Library
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The RPCI-81 Canine BAC Library has been constructed ion our
laboratory by Robin Li (Stanford University). The library
constructed was collaborative effort that included support
from the following groups: Emmanual Mignot (Stanford University),
Donald Patterson (University of Pennsylvania), Gus Agiurre
(Cornell University), Elaine Ostrander (University of Washington,
Seattle), and Bernard van Oost (University of Utrecht, the
Netherlands).
Male Doberman Pinscher dog blood genomic DNA, partially digested
with EcoRI/EcoRI Methylase, was cloned into the pBACe3.6
vector. The ligation products were transformed into
DH10B electrocompetent cells (BRL Life Technologies).
The library has been arrayed into 384-well microtiter dishes
and gridded onto 22x22cm
nylon high density hybridization filters for screening
purposes.
The Total RPCI-81 Canine BAC Library:
| Cloning
Vector |
DNA |
Plate Numbers |
Total Plates |
Total Clones Picked |
Total Empty Wells |
% Empty Wells |
| pBACe3.6 |
Doberman Pinscher (Blood) |
1-432 |
432 |
165888 |
5473 |
3.3 |
| Total Non-Recombinant
Clones |
% Non-Recombinant Clones |
Total Clones |
Average Insert Size
(Kb) |
Genomic Coverage |
| 3318 |
2.0 |
157255 |
155 |
8.1 |
click here
for a legend of the previous tables.
The average insert size
data and clone size distribution has been determined by Pulsed
Field Gel Electriphoresis analyses of random clones chosen
from library plates spanning the entire RPCI-81 library.
Further (in depth) characterization is ongoing and data will
be updated on this WebPage periodically.
The library is available as library plates, individual clones, and high-density
hybridization sets (see Filter Availability)
Individual clones identified after screening can be obtained as stab cultures
at a cost of $80 per clone in addition to a $35 set-up charge per shipment. Shipping will be done by Federal Express and charged to the recipients account
number.
With respect to the scheduling of shipments of arrayed library copies, we would
like to make you aware of our policy for
array distribution.
Please visit the ordering information
page if you want to place an order.
Academic and commercial users interested in a copy of the BAC library should
contact Pieter J. de Jong (pdejong@chori.org, fax: (510) 450-7924). For hybridization
membranes, please contact BACPAC Resources (BACPACorders@chori.org).
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