| RPCI
- 43 Male Chimpanzee BAC Library
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The RPCI-43 BAC Library has been
constructed in our laboratory by Chung-Li Shu. DNA was isolated
from white blood cells obtained from a male chimpanzee (Pan
troglodytes, "Donald", blood collection date: 9-11-97).
The blood sample from Donald (# CO551, birthdate: 10-15-1987)
was obtained from the Yerkes Non-Human Primate Center in Atlanta,
GA. The agarose-embedded DNA was partially digested with a
combination of EcoRI and EcoRI Methylase. Size selected
DNA was cloned into the pBACe3.6
vector between the EcoRI sites. The ligation products
were transformed into DH10B electrocompetent cells (BRL Life
Technologies). The library has been arrayed into 384-well
microtiter dishes and also gridded onto 22x22cm
nylon high-density filters for screening by probe hybridization.
Each hybridization membrane represents over 18,000 distinct
chimpanzee BAC clones, stamped in duplicate.
The Total RPCI-43
Male Chimpanzee BAC Library:
| Segment |
Cloning
Vector |
DNA |
Plate Numbers |
Total Plates |
Total Clones |
Total Empty Wells |
| 1 |
pBACe3.6 |
Male WBC |
1-185 |
185 |
68721 |
2319 |
| Segment |
(%) Empty Wells |
Total Non-Recombinant
Clones |
% Non-Recombinant Clones |
Average Insert Size
(Kb) |
Genomic Coverage |
| 1 |
3.3 |
approx. 960 |
1.3 |
157 |
3.5X |
click here
for a legend of the previous tables.
The total library
should represent approximately 3.5-fold total genomic representation.
The RPCI-43 Male
Chimpanzee BAC Library is available both as library plates
and high-density hybridization filter sets.
Data on the RPCI-43
clone average insert size has
been determined by Pulsed Field Gel Electriphoresis.
Clone size distribution has been plotted graphically.
Further (in depth) characterization of the library is ongoing
in our lab and data will be updated on our web page periodically.
The library is available as library plates, individual clones, and high-density
hybridization sets (see Filter Availability)
The library is available for academic users by reimbursement of duplication
costs (labor & materials), resulting in a price of $20 per 384-well dish.
Dishes can only be obtained per complete library
($3,700).Individual clones identified after screening can be obtained as stab cultures
at a cost of $25 per clone in addition to a $35 set-up charge per shipment. Shipping will be done by Federal Express and charged to the recipients account
number.
With respect to the scheduling of shipments of arrayed library copies, we would
like to make you aware of our policy for
array distribution.
Please visit the ordering information
page if you want to place an order.
Academic and commercial users interested in a copy of the BAC library should
contact Pieter J. de Jong (pdejong@chori.org, fax: (510) 450-7924). For hybridization
membranes, please contact BACPAC Resources (BACPACorders@chori.org).
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