| RPCI
- 24 Mouse (C57BL/6J Male) BAC Library |
 |
The RPCI-24 BAC library has been constructed in our laboratory
from tissues derived from single male C57BL/6J mouse. Spleen
and brain genomic DNA samples were isolated and partially
digested with MboI. Size-selected MboI fragments were cloned
into the pTARBAC1 vector between the BamHI sites. The ligation
products were transformed into DH10B electrocompetent cells
(BRL Life Technologies). The library has been arrayed into
384-well microtiter dishes and also gridded onto 22x22cm
nylon high-density filters for screening by probe hybridization.
Each hybridization membrane represents over 18,000 distinct
mouse BAC clones, stamped in duplicate.
The RPCI - 24 Male (C57BL/6J) Mouse BAC Library
| Segments |
Cloning
Vector |
DNA |
Restriction
enzyme |
Plate Numbers |
Total Plates |
Empty Wells |
Empty Wells
(%) |
| 1 |
pTARBAC1 |
Spleen |
MboI |
1-161, 168-177
|
288 |
3464 |
3.1 |
| Brain |
MboI |
162-167, 178-288
|
| 2 |
pTARBAC1 |
Brain |
MboI |
289-313 |
288 |
3951 |
3.6 |
| Spleen |
MboI |
314-576 |
| Total Library |
|
|
|
|
576 |
7415 |
3.35 |
| Segments |
Non-insert Clones (%) |
Non-insert clones |
Non-Recombinant
Clones (pUC) |
Recombinant Clones |
Average Insert Size |
Redundancy
(Genome size:
3,000 Mb) |
| 1 |
4.3 |
4607 |
1 |
102,521 |
160 Kbp |
5.5 |
| 2 |
0.5 |
533 |
0 |
106,108 |
150 Kbp |
5.3 |
| Total Library |
2.4 |
5140 |
1 |
208,629 |
155 Kbp |
10.8 |
click here
for a legend of the previous tables.
Data on the RPCI-24 clone average insert size (Segment
1, Segment
2) has been determined by Pulsed Field Gel Electrophoresis.
Clone size distribution has been plotted graphically.
The library is available as library plates, individual clones, and high-density
hybridization sets (see Filter Availability)
The library is available for academic users by reimbursement of duplication
costs (labor & materials), resulting in a price of $20 per 384-well dish.
Dishes can only be obtained per complete library
($11,520)
or per segment
($5,760)Individual clones identified after screening can be obtained as stab cultures
at a cost of $25 per clone in addition to a $35 set-up charge per shipment. Shipping will be done by Federal Express and charged to the recipients account
number.
With respect to the scheduling of shipments of arrayed library copies, we would
like to make you aware of our policy for
array distribution.
Please visit the ordering information
page if you want to place an order.
Academic and commercial users interested in a copy of the BAC library should
contact Pieter J. de Jong (pdejong@chori.org, fax: (510) 450-7924). For hybridization
membranes, please contact BACPAC Resources (BACPACorders@chori.org).
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