Cloning site: MboI (GATC); insert is underlined
--------T7------à
TACATTATACGAAGTTATATTCGATGCGGCCGCTAATACGACTCACTATAGGGAGAAGCTTGGATC
NNNNNNNNNNNNNNNNGATCCTATAGTGTCACCTAAATCGTATGCGGCCGCCCGGGCCGTCGACCA
ß-----Sp6-------
1
GATCCTATAG TGTCACCTAA ATCGTATGCG GCCGCCCGGG CCGTCGACCA
51 ATTCTCATGT TTGACAGCTT ATCATCGAAT TTCTGCCATT
CATCCGCTTA
101 TTATCACTTA TTCAGGCGTA GCAACCAGGC GTTTAAGGGC
ACCAATAACT
151 GCCTTAAAAA AATTACGCCC CGCCCTGCCA CTCATCGCAG
TACTGTTGTA
201 ATTCATTAAG CATTCTGCCG ACATGGAAGC CATCACAAAC
GGCATGATGA
251 ACCTGAATCG CCAGCGGCAT CAGCACCTTG TCGCCTTGCG
TATAATATTT
301 GCCCATGGTG AAAACGGGGG CGAAGAAGTT GTCCATATTG
GCCACGTTTA
351 AATCAAAACT GGTGAAACTC ACCCAGGGAT TGGCTGAGAC
GAAAAACATA
401 TTCTCAATAA ACCCTTTAGG GAAATAGGCC AGGTTTTCAC
CGTAACACGC
451 CACATCTTGC GAATATATGT GTAGAAACTG CCGGAAATCG
TCGTGGTATT
501 CACTCCAGAG CGATGAAAAC GTTTCAGTTT GCTCATGGAA
AACGGTGTAA
551 CAAGGGTGAA CACTATCCCA TATCACCAGC TCACCGTCTT
TCATTGCCAT
601 ACGGAATTCC GGATGAGCAT TCATCAGGCG GGCAAGAATG
TGAATAAAGG
651 CCGGATAAAA CTTGTGCTTA TTTTTCTTTA CGGTCTTTAA
AAAGGCCGTA
701 ATATCCAGCT GAACGGTCTG GTTATAGGTA CATTGAGCAA
CTGACTGAAA
751 TGCCTCAAAA TGTTCTTTAC GATGCCATTG GGATATATCA
ACGGTGGTAT
801 ATCCAGTGAT TTTTTTCTCC ATTTTAGCTT CCTTAGCTCC
TGAAAATCTC
851 GATAACTCAA AAAATACGCC CGGTAGTGAT CTTATTTCAT
TATGGTGAAA
901 GTTGGAACCT CTTACGTGCC GATCAACGTC TCATTTTCGC
CAAAAGTTGG
951 CCCAGGGCTT CCCGGTATCA ACAGGGACAC CAGGATTTAT
TTATTCTGCG
1001 AAGTGATCTT CCGTCACAGG TATTTATTCG CGATAAGCTC
ATGGAGCGGC
1051 GTAACCGTCG CACAGGAAGG ACAGAGAAAG CGCGGATCTG
GGAAGTGACG
1101 GACAGAACGG TCAGGACCTG GATTGGGGAG GCGGTTGCCG
CCGCTGCTGC
1151 TGACGGTGTG ACGTTCTCTG TTCCGGTCAC ACCACATACG
TTCCGCCATT
1201 CCTATGCGAT GCACATGCTG TATGCCGGTA TACCGCTGAA
AGTTCTGCAA
1251 AGCCTGATGG GACATAAGTC CATCAGTTCA ACGGAAGTCT
ACACGAAGGT
1301 TTTTGCGCTG GATGTGGCTG CCCGGCACCG GGTGCAGTTT
GCGATGCCGG
1351 AGTCTGATGC GGTTGCGATG CTGAAACAAT TATCCTGAGA
ATAAATGCCT
1401 TGGCCTTTAT ATGGAAATGT GGAACTGAGT GGATATGCTG
TTTTTGTCTG
1451 TTAAACAGAG AAGCTGGCTG TTATCCACTG AGAAGCGAAC
GAAACAGTCG
1501 GGAAAATCTC CCATTATCGT AGAGATCCGC ATTATTAATC
TCAGGAGCCT
1551 GTGTAGCGTT TATAGGAAGT AGTGTTCTGT CATGATGCCT
GCAAGCGGTA
1601 ACGAAAACGA TTTGAATATG CCTTCAGGAA CAATAGAAAT
CTTCGTGCGG
1651 TGTTACGTTG AAGTGGAGCG GATTATGTCA GCAATGGACA
GAACAACCTA
1701 ATGAACACAG AACCATGATG TGGTCTGTCC TTTTACAGCC
AGTAGTGCTC
1751 GCCGCAGTCG AGCGACAGGG CGAAGCCCTC GAGTGAGCGA
GGAAGCACCA
1801 GGGAACAGCA CTTATATATT CTGCTTACAC ACGATGCCTG
AAAAAACTTC
1851 CCTTGGGGTT ATCCACTTAT CCACGGGGAT ATTTTTATAA
TTATTTTTTT
1901 TATAGTTTTT AGATCTTCTT TTTTAGAGCG CCTTGTAGGC
CTTTATCCAT
1951 GCTGGTTCTA GAGAAGGTGT TGTGACAAAT TGCCCTTTCA
GTGTGACAAA
2001 TCACCCTCAA ATGACAGTCC TGTCTGTGAC AAATTGCCCT
TAACCCTGTG
2051 ACAAATTGCC CTCAGAAGAA GCTGTTTTTT CACAAAGTTA
TCCCTGCTTA
2101 TTGACTCTTT TTTATTTAGT GTGACAATCT AAAAACTTGT
CACACTTCAC
2151 ATGGATCTGT CATGGCGGAA ACAGCGGTTA TCAATCACAA
GAAACGTAAA
2201 AATAGCCCGC GAATCGTCCA GTCAAACGAC CTCACTGAGG
CGGCATATAG
2251 TCTCTCCCGG GATCAAAAAC GTATGCTGTA TCTGTTCGTT
GACCAGATCA
2301 GAAAATCTGA TGGCACCCTA CAGGAACATG ACGGTATCTG
CGAGATCCAT
2351 GTTGCTAAAT ATGCTGAAAT ATTCGGATTG ACCTCTGCGG
AAGCCAGTAA
2401 GGATATACGG CAGGCATTGA AGAGTTTCGC GGGGAAGGAA
GTGGTTTTTT
2451 ATCGCCCTGA AGAGGATGCC GGCGATGAAA AAGGCTATGA
ATCTTTTCCT
2501 TGGTTTATCA AACGTGCGCA CAGTCCATCC AGAGGGCTTT
ACAGTGTACA
2551 TATCAACCCA TATCTCATTC CCTTCTTTAT CGGGTTACAG
AACCGGTTTA
2601 CGCAGTTTCG GCTTAGTGAA ACAAAAGAAA TCACCAATCC
GTATGCCATG
2651 CGTTTATACG AATCCCTGTG TCAGTATCGT AAGCCGGATG
GCTCAGGCAT
2701 CGTCTCTCTG AAAATCGACT GGATCATAGA GCGTTACCAG
CTGCCTCAAA
2751 GTTACCAGCG TATGCCTGAC TTCCGCCGCC GCTTCCTGCA
GGTCTGTGTT
2801 AATGAGATCA ACAGCAGAAC TCCAATGCGC CTCTCATACA
TTGAGAAAAA
2851
GAAAGGCCGC CAGACGACTC ATATCGTATT TTCCTTCCGC GATATCACTT
2901 CCATGACGAC AGGATAGTCT GAGGGTTATC TGTCACAGAT
TTGAGGGTGG
2951 TTCGTCACAT TTGTTCTGAC CTACTGAGGG TAATTTGTCA
CAGTTTTGCT
3001 GTTTCCTTCA GCCTGCATGG ATTTTCTCAT ACTTTTTGAA CTGTAATTTT
3051 TAAGGAAGCC AAATTTGAGG GCAGTTTGTC ACAGTTGATT
TCCTTCTCTT
3101 TCCCTTCGTC ATGTGACCTG ATATCGGGGG TTAGTTCGTC
ATCATTGATG
3151 AGGGTTGATT ATCACAGTTT ATTACTCTGA ATTGGCTATC
CGCGTGTGTA
3201 CCTCTACCTG GAGTTTTTCC CACGGTGGAT ATTTCTTCTT
GCGCTGAGCG
3251 TAAGAGCTAT CTGACAGAAC AGTTCTTCTT TGCTTCCTCG
CCAGTTCGCT
3301 CGCTATGCTC GGTTACACGG CTGCGGCGAG CGCTAGTGAT
AATAAGTGAC
3351 TGAGGTATGT GCTCTTCTTA TCTCCTTTTG TAGTGTTGCT
CTTATTTTAA
3401 ACAACTTTGC GGTTTTTTGA TGACTTTGCG ATTTTGTTGT
TGCTTTGCAG
3451 TAAATTGCAA GATTTAATAA AAAAACGCAA AGCAATGATT
AAAGGATGTT
3501 CAGAATGAAA CTCATGGAAA CACTTAACCA GTGCATAAAC
GCTGGTCATG
3551 AAATGACGAA AGCTATCGCC ATTGCACAGT TTAATGATGA
CAGCCCGGAA
3601 GCGAGGAAAA TAACCCGGCG CTGGAGAATA GGTGAAGCAG
CGGATTTAGT
3651 TGGGGTTTCT TCTCAGGCTA TCAGAGATGC CGAGAAAGCA
GGGCGACTAC
3701 CGCACCCGGA TATGGAAATT CGAGGACGGG TTGAGCAACG
TGTTGGTTAT
3751 ACAATTGAAC AAATTAATCA TATGCGTGAT GTGTTTGGTA
CGCGATTGCG
3801 ACGTGCTGAA GACGTATTTC CACCGGTGAT CGGGGTTGCT
GCCCATAAAG
3851 GTGGCGTTTA CAAAACCTCA GTTTCTGTTC ATCTTGCTCA
GGATCTGGCT
3901 CTGAAGGGGC TACGTGTTTT GCTCGTGGAA GGTAACGACC
CCCAGGGAAC
3951 AGCCTCAATG TATCACGGAT GGGTACCAGA TCTTCATATT
CATGCAGAAG
4001 ACACTCTCCT GCCTTTCTAT CTTGGGGAAA AGGACGATGT
CACTTATGCA
4051 ATAAAGCCCA CTTGCTGGCC GGGGCTTGAC ATTATTCCTT
CCTGTCTGGC
4101 TCTGCACCGT ATTGAAACTG AGTTAATGGG CAAATTTGAT
GAAGGTAAAC
4151 TGCCCACCGA TCCACACCTG ATGCTCCGAC TGGCCATTGA
AACTGTTGCT
4201 CATGACTATG ATGTCATAGT TATTGACAGC GCGCCTAACC
TGGGTATCGG
4251 CACGATTAAT GTCGTATGTG CTGCTGATGT GCTGATTGTT
CCCACGCCTG
4301 CTGAGTTGTT TGACTACACC TCCGCACTGC AGTTTTTCGA
TATGCTTCGT
4351 GATCTGCTCA AGAACGTTGA TCTTAAAGGG TTCGAGCCTG
ATGTACGTAT
4401 TTTGCTTACC AAATACAGCA ATAGTAATGG CTCTCAGTCC
CCGTGGATGG
4451 AGGAGCAAAT TCGGGATGCC TGGGGAAGCA TGGTTCTAAA
AAATGTTGTA
4501 CGTGAAACGG ATGAAGTTGG TAAAGGTCAG ATCCGGATGA
GAACTGTTTT
4551 TGAACAGGCC ATTGATCAAC GCTCTTCAAC TGGTGCCTGG
AGAAATGCTC
4601 TTTCTATTTG GGAACCTGTC TGCAATGAAA TTTTCGATCG
TCTGATTAAA
4651 CCACGCTGGG AGATTAGATA ATGAAGCGTG CGCCTGTTAT
TCCAAAACAT
4701 ACGCTCAATA CTCAACCGGT TGAAGATACT TCGTTATCGA
CACCAGCTGC
4751 CCCGATGGTG GATTCGTTAA TTGCGCGCGT AGGAGTAATG
GCTCGCGGTA
4801 ATGCCATTAC TTTGCCTGTA TGTGGTCGGG ATGTGAAGTT
TACTCTTGAA
4851 GTGCTCCGGG GTGATAGTGT TGAGAAGACC TCTCGGGTAT
GGTCAGGTAA
4901 TGAACGTGAC CAGGAGCTGC TTACTGAGGA CGCACTGGAT
GATCTCATCC
4951 CTTCTTTTCT ACTGACTGGT CAACAGACAC CGGCGTTCGG
TCGAAGAGTA
5001 TCTGGTGTCA TAGAAATTGC CGATGGGAGT CGCCGTCGTA
AAGCTGCTGC
5051 ACTTACCGAA AGTGATTATC GTGTTCTGGT TGGCGAGCTG
GATGATGAGC
5101 AGATGGCTGC ATTATCCAGA TTGGGTAACG ATTATCGCCC
AACAAGTGCT
5151 TATGAACGTG GTCAGCGTTA TGCAAGCCGA TTGCAGAATG
AATTTGCTGG
5201 AAATATTTCT GCGCTGGCTG ATGCGGAAAA TATTTCACGT
AAGATTATTA
5251 CCCGCTGTAT CAACACCGCC AAATTGCCTA AATCAGTTGT
TGCTCTTTTT
5301 TCTCACCCCG GTGAACTATC TGCCCGGTCA GGTGATGCAC
TTCAAAAAGC
5351 CTTTACAGAT AAAGAGGAAT TACTTAAGCA GCAGGCATCT
AACCTTCATG
5401 AGCAGAAAAA AGCTGGGGTG ATATTTGAAG CTGAAGAAGT
TATCACTCTT
5451 TTAACTTCTG TGCTTAAAAC GTCATCTGCA TCAAGAACTA
GTTTAAGCTC
5501 ACGACATCAG TTTGCTCCTG GAGCGACAGT ATTGTATAAG
GGCGATAAAA
5551 TGGTGCTTAA CCTGGACAGG TCTCGTGTTC CAACTGAGTG
TATAGAGAAA
5601 ATTGAGGCCA TTCTTAAGGA ACTTGAAAAG CCAGCACCCT
GATGCGACCA
5651 CGTTTTAGTC TACGTTTATC TGTCTTTACT TAATGTCCTT
TGTTACAGGC
5701 CAGAAAGCAT AACTGGCCTG AATATTCTCT CTGGGCCCAC
TGTTCCACTT
5751 GTATCGTCGG TCTGATAATC AGACTGGGAC CACGGTCCCA
CTCGTATCGT
5801 CGGTCTGATT ATTAGTCTGG GACCACGGTC CCACTCGTAT
CGTCGGTCTG
5851 ATTATTAGTC TGGGACCACG GTCCCACTCG TATCGTCGGT
CTGATTATCA
5901 GTCTGGAACC ACGGTCCCAC TCGTATCGTC GGTCTGATTA
TTAGTCTGGG
5951 ACCACGGTCC CACTCGTATC GTCGGTCTGA TTATTAGTCT
GGGACCACGA
6001 TCCCACTCGT GTTGTCGGTC TGATTATCGG TCTGGGACCA
CGGTCCCACT
6051
TGTATTGTCG ATCAGACTAT CAGCGTGAGA CTACGATTCC ATCAATGCCT
6101 GTCAAGGGCA AGTATTGACA TGTCGTCGTA ACCTGTAGAA
CGGAGTAACC
6151 TCGGTGTGCG GTTGTATGCC TGCTGTGGAT TGCTGCTGTG
TCCTGCTTAT
6201 CCACAACATT TTGCGCACGG TTATGTGGAC AAAATACCTG GTTACCCAGG
6251 CCGTGCCGGC ACGTTAACCG GGCTGCATCC GATGCAAGTG
TGTCGCTGTC
6301 GACGAGCTCG CGAGCTCGGA CATGAGGTTG CCCCGTATTC
AGTGTCGCTG
6351 ATTTGTATTG TCTGAAGTTG TTTTTACGTT AAGTTGATGC
AGATCAATTA
6401 ATACGATACC TGCGTCATAA TTGATTATTT GACGTGGTTT
GATGGCCTCC
6451 ACGCACGTTG TGATATGTAG ATGATAATCA TTATCACTTT
ACGGGTCCTT
6501 TCCGGTGATC CGACAGGTTA CGGGGCGGCG ACCTCGCGGG
TTTTCGCTAT
6551 TTATGAAAAT TTTCCGGTTT AAGGCGTTTC CGTTCTTCTT
CGTCATAACT
6601 TAATGTTTTT ATTTAAAATA CCCTCTGAAA AGAAAGGAAA
CGACAGGTGC
6651 TGAAAGCGAG CTTTTTGGCC TCTGTCGTTT CCTTTCTCTG
TTTTTGTCCG
6701 TGGAATGAAC AATGGAAGTC CGAGCTCATC GCTAATAACT
TCGTATAGCA
6751 TACATTATAC GAAGTTATAT TCGATGCGGC CGCTAATACG
ACTCACTATA
6801 GGGAGAAGCT TGGATC
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