Mapped clones information
on the project
For the purpose of integrating
cytogenetic, radiation hybrid, linkage and sequence
maps of the human genome, as well as for research on
cancer, a number of clones have been selected for and
mapped by Fluorescent in-situ Hybridization at a 1Mb
resolution. This set of clones, which mostly contains
clones from our human library (RPCI-11) and clones from
the Caltech Human Libraries is also known as the HBRC_1
set (Human BAC Resource Consortium_1 set).
has been carried out through a collaboration of several
different groups and coordinated by Barbara J.Trask
of cytogenetic landmarks into the draft sequence of
the human genome, The BAC Resource consortium, Nature
409, 953-958 (2001)). Funding for the project was provided
by the National Cancer Institute. Our Resources &
Research Section provides more
information on the project, which brought about
the construction of these resources.
A complete and updated list is
available from the
National Center for Biotechnology Information's website.
About a third (3967) of these clones are currently distributed
by CHORI BACPAC Resources.
Clones from this collection
can be ordered individually through our online
ordering system by requesting the particular clone
while specifying in the "comment section"
of the requisition: "PLEASE PROVIDE THE FISH-CONFIRMED
VERSION OF THIS CLONE". The clones are also available
by ordering the entire collection (in deepwell blocks)
or by ordering the entire collections specific for particular
chromosomes. Such orders cannot be placed online and
require an email communication with BACPACorders@chori.org.
The entire collection is contained in forty-six 96 deepwell
blocks, which have been arranged according to chromosome
order. Clones from duplicated regions (hybridizing to
mutiple chromosomes) are called "multisite clones"
and these clones have been arrayed into separate blocks.
Due to regular
updates of the complete list, and the possibility of
future reorganization of the collection in the blocks,
a release number - currently 1.3 - was assigned to it.
Please consult the Resources and Research section of
our site (http://bacpac.chori.org/mapped-clones.htm)
to see the content of these collections.
The collection is also subject
to an ongoing quality control effort in our facilities
ensuring the quality and reliability of our FISH Mapped
Clone resource. Please contact our BACPAC manager
for more details related to the quality assessment.
The clones or derived DNA samples shall not be transferred
to others, shared with the other laboratories or used in commercial
enterprises. For commercial use, a Materials Transfer Agreements
will need to be executed with the owners of the library clones
used (e.g. Roswell Park Cancer Institute in the case of RPCI-11
or -13 BAC clones.
There is no warranty
of suitability of the DNA preparation for the user's conceivable
application. While we don't assume financial liabilities,
it is recommended to order "192 DNA samples" ($384
plus $35 set-up fee) at first to test whether quality and
quantity of DNA is suitable for the user's application prior
to ordering the whole set of DNA sample.
For more details
please view the complete Distribution
Pricing includes labor,
reagents, reasonable part of the equipment usage, maintenance
costs and institutional overhead charges. Plates can
only be ordered by subsets of chromosome (2 to 3 plates).
|41st and above
For pricing and
J. de Jong
prep FISH confirmed clones
DNA in chromosome specific
and shipping details, please view the ordering
and pricing information page.