| RPCI
- 32 Male (Brown Norway) Rat BAC Library |
 |
The RPCI-32 Male Brown Norway Rat
BAC Library has been constructed on our laboratory by Chung-Li
Shu. Library construction was supported by a sub-contract
from the Whitehead Institute for Biomedical Research.
Funding was from a NIH grant (5R01HL55726-03) awarded to E.
Lander (P.I.). Male Brown Norway (BN/ss/NH/sd) rat spleen
genomic DNA (partially EcoRI digested) was cloned into DH10B
electrocompetent cells (BRL Life Technologies). The
library has been arrayed into 384-well microtiter dishes and
also gridded onto 22x22cm high-density
nylon hybridization filters for screening by probe hybridization.
Each hybridization. Each hybridization membrane represents
over 18,000 distinct rat BAC clones, stamped in duplicate.
The Total RPCI-32
Rat BAC Library:
| Segment |
Cloning
Vector |
DNA |
Plate Numbers |
Total Plates |
Total Clones |
Total Empty Wells |
| 1 |
pBACe3.6 |
Male Brown Norway Rat Spleen |
1-288 |
288 |
106,648 |
1,244 |
| 2 |
pBACe3.6 |
Male Brown Norway Rat Spleen |
289-624 |
336 |
121,665 |
1,559 |
| Total Library |
|
|
1-624 |
624 |
228,313 |
2,803 |
| Segment |
(%) Empty Wells |
Total Non-Recombinant
Clones |
% Non-Recombinant Clones |
Average Insert
Size (Kb) |
Genomic Coverage |
| 1 |
1.0 |
approx. 2,700 |
2.5 |
147 |
5.2X |
| 2 |
1.2 |
approx. 5,800 |
4.5 |
153 |
6.2X |
| Total |
1.1 |
approx. 8,500 |
3.7 |
150 |
11.4X |
click here
for a legend of the previous tables.
The average
insert size data has been determined by Pulsed Field Gel
Electrophoretic analysis of clones. Further (in depth)
characterization of the library is ongoing in our lab and
data will be updated on our web page periodically.
Individual clones identified after screening can be obtained as stab cultures
at a cost of $80 per clone in addition to a $35 set-up charge per shipment. Shipping will be done by Federal Express and charged to the recipients account
number.
With respect to the scheduling of shipments of arrayed library copies, we would
like to make you aware of our policy for
array distribution.
Please visit the ordering information
page if you want to place an order.
Academic and commercial users interested in a copy of the BAC library should
contact Pieter J. de Jong (pdejong@chori.org, fax: (510) 450-7924). For hybridization
membranes, please contact BACPAC Resources (BACPACorders@chori.org).
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