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 Home > Resources > Libraries
  RPCI- 31 Female (Brown Norway) Rat PAC Library   

The RPCI-31 PAC Library has been constructed in our laboratory by Peng Yeong Woon*.   PAC library construction was funded by a grant from NIG (#1R01HL55700-02).   Female Brown Norway (BN/ssNHsd) rat brain genomic DNA (partially MboI digested) was cloned between the BamHI sites of the pPAC4 vector.  The ligation products were transformed into DH10B electrocompetent cells (BRL Life Technologies).  The library has been arrayed into 384 well microtiter dishes and also gridded onto 22x22cm nylon high density hybridization filters for screening by probe hybridization.  Each hybridization membrane represents over 18,000 distinct rat PAC clones, stamped in duplicate.

The Total RPCI-31 Rat PAC Library:

Clones Picked

Empty Wells

% Empty

Non-Recombinant Clones (%)

Total Clones

Average Insert Size

384 Well Plate Numbers

236189

4940

2.1

11283 (4.8)

215409

142.6 Kbp

1-614

 

Segments

Clones Picked

Empty Wells

% Empty

% Non -Recombinant

Total Clones

Average Insert Size

384 Well Plate Numbers

Genomic Coverage

1 126430 2775 2.2 2.5% 120494 139.0 Kbp 1-336 5.6X
2 109759 2165 2.0 7.4% 99471 147.0 Kbp 337-614 4.9X

click here for a legend of the previous tables.

The average insert size data has been determined by Pulsed Field Gel Electrophoretic analysis of clones. 

Reference: Woon, P.-Y. et al. (1998) Construction and Characterization of a 10-fold Genome Equivalent Rat P1-Derived Artificial Chromosome Library. Genomics 50: 306-316

The library is available as library plates, individual clones, and high-density hybridization sets (see Filter Availability)

Individual clones identified after screening can be obtained as stab cultures at a cost of $80 per clone in addition to a $35 set-up charge per shipment. Shipping will be done by Federal Express and charged to the recipients account number.

* Visiting scientist supported by the Wellcome Trust, permanent address:
                Peng Yeong Woon
                The Wellcome Trust For Human Genetics
                University of Oxford
                Windmill Road
                Windmill Road
                Headington OX3 7BN
                Oxford UK
                email:   woon@well.ox.ac.uk

With respect to the scheduling of shipments of arrayed library copies, we would like to make you aware of our policy for array distribution.

Please visit the ordering information page if you want to place an order.

Academic and commercial users interested in a copy of the BAC library should contact Pieter J. de Jong (pdejong@chori.org, fax: (510) 450-7924). For hybridization membranes, please contact BACPAC Resources (BACPACorders@chori.org).

 

 

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