| RPCI-
31 Female (Brown Norway) Rat PAC Library |
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The RPCI-31 PAC Library has been constructed in our laboratory
by Peng Yeong Woon*. PAC library construction was funded
by a grant from NIG (#1R01HL55700-02). Female Brown
Norway (BN/ssNHsd) rat brain genomic DNA (partially MboI digested)
was cloned between the BamHI sites of the pPAC4
vector. The ligation products were transformed into
DH10B electrocompetent cells (BRL Life Technologies).
The library has been arrayed into 384 well microtiter dishes
and also gridded onto 22x22cm nylon high
density hybridization filters for screening by probe hybridization.
Each hybridization membrane represents over 18,000 distinct
rat PAC clones, stamped in duplicate.
The Total RPCI-31 Rat PAC Library:
Clones Picked |
Empty Wells |
% Empty |
Non-Recombinant
Clones (%) |
Total Clones |
Average Insert Size |
384 Well Plate Numbers |
236189 |
4940 |
2.1 |
11283 (4.8) |
215409 |
142.6 Kbp |
1-614 |
Segments |
Clones Picked |
Empty Wells |
% Empty |
% Non -Recombinant |
Total Clones |
Average Insert Size |
384 Well Plate Numbers |
Genomic Coverage |
| 1 |
126430 |
2775 |
2.2 |
2.5% |
120494 |
139.0 Kbp |
1-336 |
5.6X |
| 2 |
109759 |
2165 |
2.0 |
7.4% |
99471 |
147.0 Kbp |
337-614 |
4.9X |
click here
for a legend of the previous tables.
The average insert size data
has been determined by Pulsed Field Gel Electrophoretic analysis
of clones.
Reference:
Woon, P.-Y. et al. (1998) Construction and Characterization
of a 10-fold Genome Equivalent Rat P1-Derived Artificial Chromosome
Library. Genomics 50: 306-316
The library is available as library plates, individual clones, and high-density
hybridization sets (see Filter Availability)
The library is available for academic users by reimbursement of duplication
costs (labor & materials), resulting in a price of $20 per 384-well dish.
Dishes can only be obtained per complete library
($12,280)
or per segment (#1:
$6,720, #2: $5,560). Individual clones identified after screening can be obtained as stab cultures
at a cost of $25 per clone in addition to a $35 set-up charge per shipment. Shipping will be done by Federal Express and charged to the recipients account
number.
* Visiting scientist supported by the Wellcome Trust, permanent
address:
Peng Yeong Woon
The Wellcome Trust For Human Genetics
University of Oxford
Windmill Road
Windmill Road
Headington OX3 7BN
Oxford UK
email: woon@well.ox.ac.uk
With respect to the scheduling of shipments of arrayed library copies, we would
like to make you aware of our policy for
array distribution.
Please visit the ordering information
page if you want to place an order.
Academic and commercial users interested in a copy of the BAC library should
contact Pieter J. de Jong (pdejong@chori.org, fax: (510) 450-7924). For hybridization
membranes, please contact BACPAC Resources (BACPACorders@chori.org).
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